| # | Athlete | Time |
|---|---|---|
| 1 | Matthew Hudson-SmithGreat Britain | 44.10 |
| 2 | Christopher BaileyUnited States | 44.15 |
| 3 | Jacory PattersonUnited States | 44.31 |
| 4 | Khaleb McRaeUnited States | 44.45 |
| 5 | Muzala SamukongaZambia | 44.49 |
| 6 | Charlie DobsonGreat Britain | 44.65 |
| 7 | Kirani JamesGrenada | 44.80 |
| 8 | Alexander DoomBelgium | 45.68 |
Times in Sydney time (AEDT). Results: Wikipedia, “2025 Prefontaine Classic”, CC BY-SA 4.0. Unofficial, updated after the event.