| # | Athlete | Time |
|---|---|---|
| 1 | Henriette JægerNorway | 50.09 |
| 2 | Lieke KlaverNetherlands | 50.17 |
| 3 | Isabella WhittakerUnited States | 50.63 |
| 4 | Alexis HolmesUnited States | 50.73 |
| 5 | Salwa Eid NaserBahrain | 51.08 |
| 6 | Natalia BukowieckaPoland | 51.19 |
| 7 | Britton WilsonUnited States | 51.69 |
| 8 | Sada WilliamsBarbados | 53.05 |
Times in Sydney time (AEDT). Results: Wikipedia, “2025 Athletissima”, CC BY-SA 4.0. Unofficial, updated after the event.