| # | Athlete | Time |
|---|---|---|
| 1 | Charlie DobsonGreat Britain | 44.14 |
| 2 | Matthew Hudson-SmithGreat Britain | 44.27 |
| 3 | Zakithi NeneSouth Africa | 44.29 |
| 4 | Vernon NorwoodUnited States | 44.34 |
| 5 | Busang KebinatshipiBotswana | 44.51 |
| 6 | Reece HolderAustralia | 44.76 |
| 7 | Bayapo NdoriBotswana | 45.30 |
| 8 | Toby HarriesGreat Britain | 45.41 |
Times in Sydney time (AEDT). Results: Wikipedia, “2025 London Athletics Meet”, CC BY-SA 4.0. Unofficial, updated after the event.