| # | Athlete | Time |
|---|---|---|
| 1 | Isabella WhittakerUnited States | 49.58 |
| 2 | Henriette JægerNorway | 49.62 |
| 3 | Amber AnningGreat Britain | 50.24 |
| 4 | Rhasidat AdelekeIreland | 50.42 |
| 5 | Lieke KlaverNetherlands | 50.64 |
| 6 | Natalia BukowieckaPoland | 50.67 |
| 7 | Paula SevillaSpain | 50.92 |
| 8 | Sada WilliamsBarbados | 50.94 |
Times in Sydney time (AEDT). Results: Wikipedia, “2025 Bislett Games”, CC BY-SA 4.0. Unofficial, updated after the event.