| # | Athlete | Time |
|---|---|---|
| 1 | Sydney McLaughlin-LevroneUnited States | 49.43 |
| 2 | Aaliyah ButlerUnited States | 49.86 |
| 3 | Isabella WhittakerUnited States | 50.81 |
| 4 | Rhasidat AdelekeIreland | 51.33 |
| 5 | Dejanea OakleyJamaica | 51.45 |
| 6 | Rosey EffiongUnited States | 51.62 |
| 7 | Bailey LearUnited States | 51.83 |
Times in Sydney time (AEDT). Results: Wikipedia, “2025 Prefontaine Classic”, CC BY-SA 4.0. Unofficial, updated after the event.