| # | Athlete | Time |
|---|---|---|
| 1 | Femke BolNetherlands | 51.95 |
| 2 | Dalilah MuhammadUnited States | 52.58 |
| 3 | Anna CockrellUnited States | 52.91 |
| 4 | Emma ZapletalováSlovakia | 54.51 |
| 5 | Andrenette KnightJamaica | 54.54 |
| 6 | Ayomide FolorunsoItaly | 55.08 |
| 7 | Zenéy GeldenhuysSouth Africa | 55.16 |
| 8 | Gianna WoodruffPanama | 59.21 |
Times in Sydney time (AEDT). Results: Wikipedia, “2025 Herculis”, CC BY-SA 4.0. Unofficial, updated after the event.