| # | Athlete | Time |
|---|---|---|
| 1 | Marileidy PaulinoDominican Republic | 49.06 |
| 2 | Aaliyah ButlerUnited States | 49.09 |
| 3 | Nickisha PryceJamaica | 49.63 |
| 4 | Natalia BukowieckaPoland | 49.72 |
| 5 | Lieke KlaverNetherlands | 50.23 |
| 6 | Martina WeilChile | 50.30 |
| 7 | Yemi Mary JohnGreat Britain | 51.10 |
| 8 | Amandine BrossierFrance | 52.73 |
Times in Sydney time (AEDT). Results: Wikipedia, “2025 Herculis”, CC BY-SA 4.0. Unofficial, updated after the event.