| # | Athlete | Time |
|---|---|---|
| 1 | Femke BolNetherlands | 52.46 |
| 2 | Andrenette KnightJamaica | 53.90 |
| 3 | Ayomide FolorunsoItaly | 54.74 |
| 4 | Rushell ClaytonJamaica | 54.83 |
| 5 | Zenéy GeldenhuysSouth Africa | 55.37 |
| 6 | Fatoumata DialloPortugal | 55.54 |
| 7 | Naomi Van den BroeckBelgium | 55.69 |
| 8 | Cassandra TateUnited States | 56.06 |
| 9 | Rogail JosephSouth Africa | 57.91 |
Times in Sydney time (AEDT). Results: Wikipedia, “2025 Meeting International Mohammed VI d'Athlétisme de Rabat”, CC BY-SA 4.0. Unofficial, updated after the event.