| # | Athlete | Time |
|---|---|---|
| 1 | Ackera NugentJamaica | 12.32 |
| 2 | Tobi AmusanNigeria | 12.38 |
| 3 | Kendra HarrisonUnited States | 12.50 [.492] |
| 4 | Masai RussellUnited States | 12.50 [.494] |
| 5 | Danielle WilliamsJamaica | 12.51 |
| 6 | Tonea MarshallUnited States | 12.57 |
| 7 | Aaliyah McCormickUnited States | 12.98 |
| — | Tia JonesUnited States | DNF |
| — | Alia ArmstrongUnited States | DNF |
Times in Sydney time (AEDT). Results: Wikipedia, “2025 Prefontaine Classic”, CC BY-SA 4.0. Unofficial, updated after the event.