| # | Athlete | Time |
|---|---|---|
| 1 | Alison dos SantosBrazil | 46.65 |
| 2 | Rai BenjaminUnited States | 46.71 |
| 3 | Ezekiel NathanielNigeria | 47.88 |
| 4 | Trevor BassittUnited States | 48.29 |
| 5 | Abderrahman SambaQatar | 48.76 |
| 6 | CJ AllenUnited States | 49.58 |
| 7 | Assinie WilsonJamaica | 49.89 |
| 8 | Alessandro SibilioItaly | 50.17 |
| 9 | Malik James-KingJamaica | 50.21 |
Times in Sydney time (AEDT). Results: Wikipedia, “2025 Prefontaine Classic”, CC BY-SA 4.0. Unofficial, updated after the event.