| # | Athlete | Time |
|---|---|---|
| 1 | Andrenette KnightJamaica | 53.67 |
| 2 | Ayomide FolorunsoItaly | 54.21 |
| 3 | Rushell ClaytonJamaica | 54.31 |
| 4 | Lina NielsenGreat Britain | 54.66 |
| 5 | Louise MaravalFrance | 54.86 |
| 6 | Zenéy GeldenhuysSouth Africa | 55.29 |
| 7 | Shiann SalmonJamaica | 55.47 |
| 8 | Linda OlivieriItaly | 56.06 |
| 9 | Cassandra TateUnited States | 56.15 |
Times in Sydney time (AEDT). Results: Wikipedia, “2025 Golden Gala”, CC BY-SA 4.0. Unofficial, updated after the event.